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Taken together, we diagnosed the patient with both pernicious anemia due to vitamin B 12 deficiency caused by autoimmune gastritis, and thalassemia

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39 For immunohistochemistry, the tissue sections were sequentially placed in xylene I for 15 mins, xylene II for 15 mins, anhydrous ethanol I for 5 mins, anhydrous ethanol II for 5 mins, 85% alcohol for 5 mins, and 75% alcohol for 5 mins, and then were finally washed using distilled water