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There are two main processes by which peptide bonds can be degraded: Hydrolysis Enzymatic Hydrolysis Acid or Alkaline Hydrolysis Chemical Methods Edman Degradation Cyanogen Bromide (CNBr) Cleavage Hydroxylamine Cleavage Hydrolysis of Peptide Bonds The hydrolysis of a peptide bond is a process in which a peptide bond is cleaved by adding a water molecule