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In vivo intestinal permeability measurements Once every week, mice were housed in metabolic cages after a 4 h fasting of food and water and immediately after a gavage of 0.2 ml of a 10 ml sugar probe containing 100 mg of sucrose, 12 mg of lactulose, 8 mg of mannitol and 6 mg of sucralose

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This process is ongoing—which is why hair loss continues without intervention