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AlphaFold predicted LYSET to be a three-transmembrane (TM) helices-containing protein 18 , while other prediction tools, such as TOPCONS and OCTOPUS, suggested it had two-TM helices 19,20

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Starvation, amino acid deprivation, or growth factor release, by removing the mTORC1 restraint, allows Ulk1 to promote autophagy