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We chose a highly parallel protein complementation assay 27 , in which a methotrexate-resistant murine dihydrofolate reductase (DHFR) is split into two fragments (‘DH’ and ‘FR’), which are fused to bait and prey proteins

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FR-α is also present in the choroid plexus, which has the important function of supplying folate to the central nervous system (76, 77)