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Zebrafish fibroblast cell line ZF4 Embryonic zebrafish fibroblast cell line ZF4 25 (CVCL_3275) was cultured in Dulbecco’s modified Eagle’s medium/Ham’s Nutrient Mixture F-12 (DMEM:F12) containing phenol red (Gibco, Paisley, UK), supplemented with 10% fetal bovine serum (Gibco, Paisley, UK), 1% penicillin-streptomycin 100 U/mL (Gibco, Paisley, UK), 2.5 mM L-glutamine (Lonza, Basel, Swiss), 15 mM HEPES (Gibco, Paisley, UK), 0.5 mM sodium pyruvate (Sigma-Aldrich, Steinheim, Germany), and 1200 mg/L sodium bicarbonate (Gibco, Paisley, UK)