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EAE in bone marrow transplanted male animals was induced in anesthetized 8–10-week-old animals after irradiation by subcutaneous injection at the tail base of 10 μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO)
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This activates mechanotransduction mediated by Yes-associated protein, which significantly upregulates the expression of alkaline phosphatase, osteocalcin, and osteopontin, thereby accelerating osseointegration
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