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To understand the catalytic mechanism of tyrosinase in detail, we determined the cryo-trapped crystal structures at very high resolutions, which suggest an important new step for the tyrosinase reaction: the hydroxylation reaction triggered by the movement of Cu A , which induces the syn-to-anti rearrangement of the copper ligands after the formation of μ-η 2 :η 2 -peroxo-dicopper(II) core