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Briefly, samples were thawed, centrifuged at 800 g for 15 min at room temperature, and tested in separate assays measuring IgA and IgG

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The gene encoding PIS (NCBI NP_006310.1) was amplified from a cDNA clone (#BC001444) using primer pairs 5′-GGGAATATTtaaaaaATGtctggatccCCAGACGAAAATATCTTCCTG-3′ and 5′-gccctgaaacagcacttccagggtaccCTTCTTCTTGGCGCGGTCTGC-3′